Coding
GuCHI1

Part:BBa_K4947027:Design

Designed by: Williams Liu   Group: iGEM23_Yale   (2023-10-12)


G. uralensis Chalcone Isomerase 1 Codon-Optimized CDS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 37
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 37
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 37
    Illegal NgoMIV site found at 60
    Illegal NgoMIV site found at 94
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon optimization and domestication was done to improve recombinant expression in E. coli and enable restriction enzyme-based swapping of promoters and terminators, respectively.

Source

This gene homolog that encodes for CHI was chosen rationally after thorough literature review. CHI is an enzyme involved in the biosynthetic pathway of daidzein, converting isoliquiritigenin into liquiritigenin, both stable intermediates. The gene sequence was sourced from NCBI GenBank [1], and produced by Twist Bioscience.

References

1. https://www.ncbi.nlm.nih.gov/protein/ABM66533.1

2. View our contributions page (https://2023.igem.wiki/yale/contribution) for a spreadsheet of all our sources!

3. Yan, Yin., et al. (2020). De novo biosynthesis of liquiritin in Saccharomyces cerevisiae. NCBI. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161706/

4. Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome. Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ. Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 10.1016/j.cell.2022.03.008. Epub 2022 Apr 1. 10.1016/j.cell.2022.03.008 PubMed 35366417